› 2zgo

X-ray diffraction
2Å resolution

Crystal structure of AAL mutant H59Q complex with lactose

Released:
DOI: 10.2210/pdb2zgo/pdb
PDB entry 2zgo coloured by chain, front view.
PDB entry 2zgo coloured by chain, side view.
PDB entry 2zgo coloured by chain, top view.
Source organism: Cyclocybe aegerita
Primary publication:
Structural basis for the tumor cell apoptosis-inducing activity of an antitumor lectin from the edible mushroom Agrocybe aegerita.
Yang N, Li DF, Feng L, Xiang Y, Liu W, Sun H, Wang DC
J Mol Biol 387 694-705 (2009)
PMID: 19361423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Anti-tumor lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 18.17 KDa
Source organism: Cyclocybe aegerita
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6WY08 (Residues: 1-158; Coverage: 100%)
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P1
Unit cell:
a: 40.66Å b: 42.85Å c: 55.97Å
α: 101.88° β: 98.72° γ: 117.24°
R-values:
R R work R free
0.2 0.2 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Bioactive proteins from mushrooms.
Xu et al. (2011)
6 more

1 mention without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)