› 2zi0

X-ray diffraction
2.82Å resolution

Crystal structure of Tav2b/siRNA complex

Released:
DOI: 10.2210/pdb2zi0/pdb
PDB entry 2zi0 coloured by chain, front view.
PDB entry 2zi0 coloured by chain, side view.
PDB entry 2zi0 coloured by chain, top view.
Source organism: Tomato aspermy virus
Primary publication:
Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b.
Chen HY, Yang J, Lin C, Yuan YA
EMBO Rep 9 754-60 (2008)
PMID: 18600235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-186534 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Suppressor of silencing 2b Chains: A, B
Molecule details ›
Chains: A, B
Length: 75 amino acids
Theoretical weight: 9.14 KDa
Source organism: Tomato aspermy virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8UYT3 (Residues: 1-69; Coverage: 73%)
Gene name: ORF2b
Sequence domains: Cucumovirus protein 2B
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
RNA (5'-D(P*AP*GP*AP*CP*AP*GP*CP*AP*UP*UP*AP*UP*GP*CP*UP*GP*UP*CP*UP*UP*U)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 21 nucleotides
Theoretical weight: 6.65 KDa
Source organism: Tomato aspermy virus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 86.455Å b: 122.043Å c: 28.193Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.276
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

RNA silencing suppression by plant pathogens: defence, counter-defence and counter-counter-defence.
Pumplin et al. (2013)
13 more

15 mentions without citation

Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b.
Chen et al. (2008)
14 more