› 2zii

X-ray diffraction
3.05Å resolution

Crystal Structure of Yeast Vps74-N-term Truncation Variant

Released:
DOI: 10.2210/pdb2zii/pdb
PDB entry 2zii coloured by chain, front view.
PDB entry 2zii coloured by chain, side view.
PDB entry 2zii coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Golgi localization of glycosyltransferases requires a Vps74p oligomer.
Schmitz KR, Liu J, Li S, Setty TG, Wood CS, Burd CG, Ferguson KM
Dev Cell 14 523-34 (2008)
PMID: 18410729

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 74 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 288 amino acids
Theoretical weight: 33.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06385 (Residues: 60-345; Coverage: 83%)
Gene names: D9481.15, VPS74, YDR372C
Sequence domains: Golgi phosphoprotein 3 (GPP34)
Structure domains: yeast vps74-n-term truncation variant domain like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P3121
Unit cell:
a: 104.081Å b: 104.081Å c: 292.759Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.22 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

46 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
45 more

1 mention without citation

Golgi localization of glycosyltransferases requires a Vps74p oligomer.
Schmitz et al. (2008)