› 2zj2

X-ray diffraction
2.4Å resolution

Archaeal DNA helicase Hjm apo state in form 1

Released:
DOI: 10.2210/pdb2zj2/pdb
PDB entry 2zj2 coloured by chain, front view.
PDB entry 2zj2 coloured by chain, side view.
PDB entry 2zj2 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Atomic structures and functional implications of the archaeal RecQ-like helicase Hjm.
Oyama T, Oka H, Mayanagi K, Shirai T, Matoba K, Fujikane R, Ishino Y, Morikawa K
BMC Struct Biol 9 2 (2009)
PMID: 19159486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase Hel308 Chain: A
Molecule details ›
Chain: A
Length: 720 amino acids
Theoretical weight: 82.75 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: O73946 (Residues: 1-720; Coverage: 100%)
Gene names: PF0677, hel308, hjm
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 117.269Å b: 83.441Å c: 95.019Å
α: 90° β: 120.69° γ: 90°
R-values:
R R work R free
0.228 0.228 0.299
Expression system: Escherichia coli

Quick links

Citations

6 review citations

End resection at double-strand breaks: mechanism and regulation.
Symington LS. (2014)
5 more

4 mentions without citation

Ski2-like RNA helicase structures: common themes and complex assemblies.
Johnson et al. (2013)
3 more