› 2zjt

X-ray diffraction
2.8Å resolution

Crystal structure of dna gyrase B' domain sheds lights on the mechanism for T-segment navigation

Released:
DOI: 10.2210/pdb2zjt/pdb
PDB entry 2zjt coloured by chain, front view.
PDB entry 2zjt coloured by chain, side view.
PDB entry 2zjt coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of DNA gyrase B' domain sheds lights on the mechanism for T-segment navigation.
Fu G, Wu J, Liu W, Zhu D, Hu Y, Deng J, Zhang XE, Bi L, Wang DC
Nucleic Acids Res 37 5908-16 (2009)
PMID: 19596812

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.94 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WG45 (Residues: 448-675; Coverage: 34%)
Gene names: MTCY10H4.03, Rv0005, gyrB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A, RIGAKU
Spacegroup: P212121
Unit cell:
a: 52.831Å b: 52.763Å c: 192.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.28
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Exploiting bacterial DNA gyrase as a drug target: current state and perspectives.
Collin et al. (2011)
6 more

4 mentions without citation

Structural insights into the quinolone resistance mechanism of Mycobacterium tuberculosis DNA gyrase.
Piton et al. (2010)
3 more