› 2zk7

X-ray diffraction
2.71Å resolution

Structure of a C-terminal deletion mutant of Thermoplasma acidophilum aldohexose dehydrogenase (AldT)

Released:
DOI: 10.2210/pdb2zk7/pdb
PDB entry 2zk7 coloured by chain, front view.
PDB entry 2zk7 coloured by chain, side view.
PDB entry 2zk7 coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Primary publication:
C-terminal tail derived from the neighboring subunit is critical for the activity of Thermoplasma acidophilum D-aldohexose dehydrogenase.
Nishioka T, Yasutake Y, Nishiya Y, Tamura N, Tamura T
Proteins 74 801-7 (2009)
PMID: 19089950

Function and Biology Details

Reaction catalysed: 
D-glucose + NAD(+) = D-glucono-1,5-lactone + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose 1-dehydrogenase related protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.3 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HK51 (Residues: 1-249; Coverage: 98%)
Gene name: Ta0754
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P6122
Unit cell:
a: 68.238Å b: 68.238Å c: 337.356Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.237 0.289
Expression system: Escherichia coli

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Citations

2 citation in other articles

Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV.
Nishioka et al. (2012)
1 more