› 2zku

X-ray diffraction
1.95Å resolution

Structure of hepatitis C virus NS5B polymerase in a new crystal form

Released:
DOI: 10.2210/pdb2zku/pdb
PDB entry 2zku coloured by chain, front view.
PDB entry 2zku coloured by chain, side view.
PDB entry 2zku coloured by chain, top view.
Source organism: Hepatitis C virus subtype 1b
Entry author: Biswal BK

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 576 amino acids
Theoretical weight: 64.1 KDa
Source organism: Hepatitis C virus subtype 1b
Expression system: Escherichia coli
UniProt:
  • Canonical: P26663 (Residues: 2420-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand ACY 7 x ACY
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 101.99Å b: 102.06Å c: 251.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.246
Expression system: Escherichia coli

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