› 2zld

X-ray diffraction
3Å resolution

Structure of OmpF co-crystallized with T83

Released:
DOI: 10.2210/pdb2zld/pdb
PDB entry 2zld coloured by chain, front view.
PDB entry 2zld coloured by chain, side view.
PDB entry 2zld coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the OmpF porin: function in a colicin translocon.
Yamashita E, Zhalnina MV, Zakharov SD, Sharma O, Cramer WA
EMBO J 27 2171-80 (2008)
PMID: 18636093

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136398 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Outer membrane porin F Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 37.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin
Colicin-E3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 614 Da
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P63
Unit cell:
a: 116.867Å b: 116.867Å c: 114.331Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.267 0.266 0.294
Expression system: Escherichia coli

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Citations

19 review citations

Filming biomolecular processes by high-speed atomic force microscopy.
Ando et al. (2014)
18 more

5 mentions without citation

The bacterial outer membrane β-barrel assembly machinery.
Kim et al. (2012)
4 more