› 2zmw

X-ray diffraction
2Å resolution

Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0

Released:
DOI: 10.2210/pdb2zmw/pdb
PDB entry 2zmw coloured by chain, front view.
PDB entry 2zmw coloured by chain, side view.
PDB entry 2zmw coloured by chain, top view.
Source organism: Lithophyllon concinna
Primary publication:
Structural characterization of a thiazoline-containing chromophore in an orange fluorescent protein, monomeric Kusabira Orange.
Kikuchi A, Fukumura E, Karasawa S, Mizuno H, Miyawaki A, Shiro Y
Biochemistry 47 11573-80 (2008)
PMID: 18844376

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 25.54 KDa
Source organism: Lithophyllon concinna
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6I7B2 (Residues: 1-218; Coverage: 100%)
Gene name: mKO
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CFY 4 x CFY

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P21
Unit cell:
a: 92.57Å b: 72.11Å c: 90.27Å
α: 90° β: 102.72° γ: 90°
R-values:
R R work R free
0.197 0.197 0.232
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
8 more

1 mention without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)