› 2zop

X-ray diffraction
2.1Å resolution

X-ray crystal structure of a CRISPR-associated Cmr5 family protein from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2zop/pdb
PDB entry 2zop coloured by chain, front view.
PDB entry 2zop coloured by chain, side view.
PDB entry 2zop coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
X-ray crystal structure of a CRISPR-associated RAMP module [corrected] Cmr5 protein [corrected] from Thermus thermophilus HB8.
Sakamoto K, Agari Y, Agari K, Yokoyama S, Kuramitsu S, Shinkai A
Proteins 75 528-32 (2009)
PMID: 19173314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-176137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system Cmr subunit Cmr5 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 114 amino acids
Theoretical weight: 13.08 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53W05 (Residues: 1-114; Coverage: 100%)
Gene names: TTHB164, cmr5
Sequence domains: CRISPR-associated protein (Cas_Cmr5)
Structure domains: AF1862-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: I222
Unit cell:
a: 102.122Å b: 114.801Å c: 122.845Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.235 0.249
Expression system: Escherichia coli

Quick links

Citations

4 review citations

CRISPR/Cas system and its role in phage-bacteria interactions.
Deveau et al. (2010)
3 more

9 mentions without citation

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
8 more