› 2zp9

X-ray diffraction
3.2Å resolution

The Nature of the TRAP:Anti-TRAP complex

Released:
DOI: 10.2210/pdb2zp9/pdb
PDB entry 2zp9 coloured by chain, front view.
PDB entry 2zp9 coloured by chain, side view.
PDB entry 2zp9 coloured by chain, top view.
Source organisms:
Primary publication:
The nature of the TRAP-Anti-TRAP complex.
Watanabe M, Heddle JG, Kikuchi K, Unzai S, Akashi S, Park SY, Tame JR
Proc Natl Acad Sci U S A 106 2176-81 (2009)
PMID: 19164760

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128292 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription attenuation protein MtrB Chains: A, B, F, G, K, L
Molecule details ›
Chains: A, B, F, G, K, L
Length: 81 amino acids
Theoretical weight: 8.75 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X6J6 (Residues: 1-74; Coverage: 100%)
Gene name: mtrB
Sequence domains: Tryptophan RNA-binding attenuator protein
Structure domains: TRAP-like
Tryptophan RNA-binding attenuator protein inhibitory protein Chains: C, D, E, H, I, J, M, N, O
Molecule details ›
Chains: C, D, E, H, I, J, M, N, O
Length: 53 amino acids
Theoretical weight: 5.66 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O31466 (Residues: 1-53; Coverage: 100%)
Gene names: BSU02530, rtpA, yczA
Sequence domains: Tryptophan RNA-binding attenuator protein inhibitory protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TRP 6 x TRP
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P6
Unit cell:
a: 197.134Å b: 197.135Å c: 56.658Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.305 0.303 0.325
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Analytical ultracentrifugation in structural biology.
Unzai S. (2018)
1 more

1 mention without citation

The nature of the TRAP-Anti-TRAP complex.
Watanabe et al. (2009)