2zqb

X-ray diffraction
2.49Å resolution

Crystal structure of a psychrotrophic RNaseHI variant with sextuple thermostabilizing mutations

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 158 amino acids
Theoretical weight: 17.64 KDa
Source organism: Shewanella oneidensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EE30 (Residues: 1-158; Coverage: 100%)
Gene names: SO_2560, rnhA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P41212
Unit cell:
a: 68.239Å b: 68.239Å c: 272.816Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.247
Expression system: Escherichia coli