› 2zsc

X-ray diffraction
1.3Å resolution

Tamavidin2, Novel Avidin-like Biotin-Binding Proteins from an Edible Mushroom

Released:
DOI: 10.2210/pdb2zsc/pdb
PDB entry 2zsc coloured by chain, front view.
PDB entry 2zsc coloured by chain, side view.
PDB entry 2zsc coloured by chain, top view.
Source organism: Pleurotus cornucopiae
Primary publication:
Tamavidins--novel avidin-like biotin-binding proteins from the Tamogitake mushroom.
Takakura Y, Tsunashima M, Suzuki J, Usami S, Kakuta Y, Okino N, Ito M, Yamamoto T
FEBS J 276 1383-97 (2009)
PMID: 19187241

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tamavidin2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 15.48 KDa
Source organism: Pleurotus cornucopiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B9A0T7 (Residues: 1-141; Coverage: 100%)
Gene name: tam2
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

3 bound ligands:
Ligand GOL 9 x GOL
Ligand BTN 2 x BTN
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 78.382Å b: 80.017Å c: 55.733Å
α: 90° β: 132.32° γ: 90°
R-values:
R R work R free
0.201 0.199 0.238
Expression system: Escherichia coli BL21

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Citations

1 review citation

Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.
Dundas et al. (2013)
32 other articles