› 2zsu

X-ray diffraction
2.2Å resolution

Crystal structure of spermidine synthase from Pyrococcus horikoshii OT3, P1 form

Released:
DOI: 10.2210/pdb2zsu/pdb
PDB entry 2zsu coloured by chain, front view.
PDB entry 2zsu coloured by chain, side view.
PDB entry 2zsu coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Mizutani H, Kunishima N

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine aminopropyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 280 amino acids
Theoretical weight: 32.01 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O57950 (Residues: 1-280; Coverage: 100%)
Gene names: PH0211, speE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AG3 3 x AG3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 67.617Å b: 81.842Å c: 96.653Å
α: 99.81° β: 103.13° γ: 107.85°
R-values:
R R work R free
0.209 0.207 0.248
Expression system: Escherichia coli

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