› 2zun

X-ray diffraction
2Å resolution

Functional Analysis of Hyperthermophilic Endocellulase from the Archaeon Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb2zun/pdb
PDB entry 2zun coloured by chain, front view.
PDB entry 2zun coloured by chain, side view.
PDB entry 2zun coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Kim H-W, Ishikawa K

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 5 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 458 amino acids
Theoretical weight: 51.95 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58925 (Residues: 1-458; Coverage: 100%)
Gene name: PH1171
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 162.157Å b: 58.404Å c: 138.332Å
α: 90° β: 109.22° γ: 90°
R-values:
R R work R free
0.216 0.216 0.225
Expression system: Escherichia coli BL21(DE3)

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