› 2zys

X-ray diffraction
3.1Å resolution

A. Fulgidus lipase with fatty acid fragment and chloride

Released:
DOI: 10.2210/pdb2zys/pdb
PDB entry 2zys coloured by chain, front view.
PDB entry 2zys coloured by chain, side view.
PDB entry 2zys coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structure of the alkalohyperthermophilic Archaeoglobus fulgidus lipase contains a unique C-terminal domain essential for long-chain substrate binding.
Chen CK, Lee GC, Ko TP, Guo RT, Huang LM, Liu HJ, Ho YF, Shaw JF, Wang AH
J Mol Biol 390 672-85 (2009)
PMID: 19447113

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase, putative Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 53.41 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O28511 (Residues: 1-474; Coverage: 100%)
Gene name: AF_1763
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PG4 1 x PG4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P43
Unit cell:
a: 99.552Å b: 99.552Å c: 59.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.252
Expression system: Escherichia coli

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Citations

7 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
6 more