› 2zyv

X-ray diffraction
1.81Å resolution

Crystal structure of mouse cytosolic sulfotransferase mSULT1D1 complex with PAPS/PAP and p-nitrophenol

Released:
DOI: 10.2210/pdb2zyv/pdb
PDB entry 2zyv coloured by chain, front view.
PDB entry 2zyv coloured by chain, side view.
PDB entry 2zyv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Snapshot of a Michaelis complex in a sulfuryl transfer reaction: Crystal structure of a mouse sulfotransferase, mSULT1D1, complexed with donor substrate and accepter substrate.
Teramoto T, Sakakibara Y, Liu MC, Suiko M, Kimura M, Kakuta Y
Biochem Biophys Res Commun 383 83-7 (2009)
PMID: 19344693

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1 family member D1 Chain: X
Molecule details ›
Chain: X
Length: 295 amino acids
Theoretical weight: 35.13 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q3UZZ6 (Residues: 1-295; Coverage: 100%)
Gene names: St1d1, Sult1d1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand PPS 1 x PPS
Ligand NPO 2 x NPO
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 155.241Å b: 67.793Å c: 42.904Å
α: 90° β: 105.23° γ: 90°
R-values:
R R work R free
0.18 0.179 0.203
Expression system: Escherichia coli BL21

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Citations

1 review citation

The PAPS-independent aryl sulfotransferase and the alternative disulfide bond formation system in pathogenic bacteria.
Malojcić et al. (2010)
9 other articles