2zzn

X-ray diffraction
2.95Å resolution

The complex structure of aTrm5 and tRNACys

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-176596 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA (guanine(37)-N1)-methyltransferase Trm5b Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 39.07 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58293 (Residues: 1-336; Coverage: 100%)
Gene names: MJ0883, trm5b
Sequence domains:
Structure domains:
RNA (71-MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.13 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 81.838Å b: 106.537Å c: 134.459Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.295
Expression systems:
  • Escherichia coli
  • Not provided