› 301d

X-ray diffraction
3Å resolution

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED

Released:
DOI: 10.2210/pdb301d/pdb
PDB entry 301d coloured by chain, front view.
PDB entry 301d coloured by chain, side view.
PDB entry 301d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme.
Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A
Science 274 2065-9 (1996)
PMID: 8953035

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196611 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA HAMMERHEAD RIBOZYME Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 5.17 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA HAMMERHEAD RIBOZYME Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 8.02 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P3121
Unit cell:
a: 65.9Å b: 65.9Å c: 136.77Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.258 0.297
Expression system: Not provided

Quick links

Citations

70 review citations

The chemical repertoire of natural ribozymes.
Doudna et al. (2002)
69 more

9 mentions without citation

Engineering of bacterial phytochromes for near-infrared imaging, sensing, and light-control in mammals.
Piatkevich et al. (2013)
8 more