› 316d

X-ray diffraction
3Å resolution

Selectivity of F8-actinomycin D for RNA:DNA hybrids and its anti-leukemia activity

Released:
DOI: 10.2210/pdb316d/pdb
PDB entry 316d coloured by chain, front view.
PDB entry 316d coloured by chain, side view.
PDB entry 316d coloured by chain, top view.
Source organism: Streptomyces antibioticus
Primary publication:
Selectivity of F8-actinomycin D for RNA:DNA hybrids and its anti-leukemia activity.
Takusagawa F, Takusagawa KT, Carlson RG, Weaver RF
Bioorg Med Chem 5 1197-207 (1997)
PMID: 9222513

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115989 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
8-FLUORO-ACTINOMYCIN D Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Streptomyces antibioticus
DNA (5'-D(*GP*AP*AP*GP*CP*TP*TP*C)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Streptomyces antibioticus
Expression system: Not provided

Ligands and Environments

No bound ligands
3 modified residues:
Ligand DVA 2 x DVA
Ligand SAR 2 x SAR
Ligand MVA 2 x MVA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 62.77Å b: 62.77Å c: 43.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.261
Expression system: Not provided

Quick links

Citations

4 review citations

Recognition of the unique structure of DNA:RNA hybrids.
Shaw et al. (2008)
3 more

3 mentions without citation

Protein meta-functional signatures from combining sequence, structure, evolution, and amino acid property information.
Wang et al. (2008)
2 more