› 354d

X-ray diffraction
1.5Å resolution

Structure of loop E FROM E. coli 5S RRNA

Released:
DOI: 10.2210/pdb354d/pdb
PDB entry 354d coloured by chain, front view.
PDB entry 354d coloured by chain, side view.
PDB entry 354d coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain.
Correll CC, Freeborn B, Moore PB, Steitz TA
Cell 91 705-12 (1997)
PMID: 9393863

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196615 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*CP*CP*GP*AP*UP*GP*GP*UP*AP*GP*UP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
RNA-DNA (5'-R(*GP*CP*GP*AP*GP*AP*GP*UP*AP*)-D(*DGP(S)*)-R(*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
1 modified residue:
Ligand G46 1 x G46

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 71.184Å b: 37.654Å c: 32.944Å
α: 90° β: 105.9° γ: 90°
R-values:
R R work R free
0.202 0.202 0.228
Expression system: Escherichia coli

Quick links

Citations

44 review citations

How RNA folds.
Tinoco et al. (1999)
43 more

12 mentions without citation

An RNA-centric historical narrative around the Protein Data Bank.
Westhof et al. (2021)
11 more