› 379d

X-ray diffraction
3.1Å resolution

THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE

Released:
DOI: 10.2210/pdb379d/pdb
PDB entry 379d coloured by chain, front view.
PDB entry 379d coloured by chain, side view.
PDB entry 379d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
The structural basis of hammerhead ribozyme self-cleavage.
Murray JB, Terwey DP, Maloney L, Karpeisky A, Usman N, Beigelman L, Scott WG
Cell 92 665-73 (1998)
PMID: 9506521

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196621 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*GP*UP*GP*GP*UP*CP*UP*GP*AP*UP*GP*AP*GP*GP*CP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 5.17 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*CP*CP*GP*AP*AP*AP*CP*UP*CP*GP*UP*AP*AP*GP*A P*GP*UP*CP*AP*CP*CP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 8.02 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CO 8 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3121
Unit cell:
a: 65.1Å b: 65.1Å c: 138.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 not available
Expression system: Not provided

Quick links

Citations

31 review citations

Functional nucleic acid sensors.
Liu et al. (2009)
30 more

3 mentions without citation

Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity.
De la Peña et al. (2003)
2 more