› 3f64

X-ray diffraction
1.95Å resolution

F17a-G lectin domain with bound GlcNAc(beta1-O)paranitrophenyl ligand

Released:
DOI: 10.2210/pdb3f64/pdb
PDB entry 3f64 coloured by chain, front view.
PDB entry 3f64 coloured by chain, side view.
PDB entry 3f64 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli.
Lonardi E, Moonens K, Buts L, de Boer AR, Olsson JD, Weiss MS, Fabre E, Guérardel Y, Deelder AM, Oscarson S, Wuhrer M, Bouckaert J
Biology (Basel) 2 894-917 (2013)
PMID: 24833052

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F17a-G fimbrial adhesin Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 19.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99003 (Residues: 23-199; Coverage: 55%)
Gene name: f17aG
Sequence domains: Fimbrial adhesin F17-AG, lectin domain
Structure domains: Bacterial adhesins - F17c-type

Ligands and Environments

1 bound ligand:
Ligand LEC 1 x LEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P6122
Unit cell:
a: 43.025Å b: 43.025Å c: 288.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.248
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Animal Enterotoxigenic Escherichia coli.
Dubreuil et al. (2016)
4 more

1 mention without citation

Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli.
Lonardi et al. (2013)