› 3zyf

X-ray diffraction
1.94Å resolution

CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH NPG AT 1.9 A RESOLUTION

Released:
DOI: 10.2210/pdb3zyf/pdb
PDB entry 3zyf coloured by chain, front view.
PDB entry 3zyf coloured by chain, side view.
PDB entry 3zyf coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
A glycopeptide dendrimer inhibitor of the galactose-specific lectin LecA and of Pseudomonas aeruginosa biofilms.
Kadam RU, Bergmann M, Hurley M, Garg D, Cacciarini M, Swiderska MA, Nativi C, Sattler M, Smyth AR, Williams P, Cámara M, Stocker A, Darbre T, Reymond JL
Angew Chem Int Ed Engl 50 10631-5 (2011)
PMID: 21919164

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PA-I galactophilic lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 122 amino acids
Theoretical weight: 12.9 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05097 (Residues: 1-122; Coverage: 100%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand 147 4 x 147
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 84.562Å b: 84.562Å c: 163.709Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Multivalent glycoconjugate syntheses and applications using aromatic scaffolds.
Chabre et al. (2013)
11 more

4 mentions without citation

Glycomimetics for the inhibition and modulation of lectins.
Leusmann et al. (2023)
3 more