› 3a01

X-ray diffraction
2.7Å resolution

Crystal structure of Aristaless and Clawless homeodomains bound to DNA

Released:
DOI: 10.2210/pdb3a01/pdb
PDB entry 3a01 coloured by chain, front view.
PDB entry 3a01 coloured by chain, side view.
PDB entry 3a01 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless.
Miyazono K, Zhi Y, Takamura Y, Nagata K, Saigo K, Kojima T, Tanokura M
EMBO J 29 1613-23 (2010)
PMID: 20389279

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116053 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox domain-containing protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 93 amino acids
Theoretical weight: 11.19 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VD99 (Residues: 169-261; Coverage: 30%)
Gene names: C15, CG7937
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
Homeobox protein aristaless Chains: B, F
Molecule details ›
Chains: B, F
Length: 67 amino acids
Theoretical weight: 8.34 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06453 (Residues: 80-146; Coverage: 16%)
Gene names: CG3935, al
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*GP*GP*CP*TP*TP*AP*AP*TP*TP*AP*AP*TP*TP*GP*CP*GP*G)-3' Chains: C, G
Molecule details ›
Chains: C, G
Length: 17 nucleotides
Theoretical weight: 5.26 KDa
5'-D(*CP*CP*GP*CP*AP*AP*TP*TP*AP*AP*TP*TP*AP*AP*GP*CP*C)-3' Chains: D, H
Molecule details ›
Chains: D, H
Length: 17 nucleotides
Theoretical weight: 5.16 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 70.93Å b: 85.4Å c: 110.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.228 0.283
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Investigating Developmental and Epileptic Encephalopathy Using Drosophila melanogaster.
Takai et al. (2020)
18 other articles

13 mentions without citation

NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation.
Aigha et al. (2020)
12 more