› 3a1p

X-ray diffraction
2.3Å resolution

Structure of Ribosome maturation protein RimM and Ribosomal protein S19

Released:
DOI: 10.2210/pdb3a1p/pdb
PDB entry 3a1p coloured by chain, front view.
PDB entry 3a1p coloured by chain, side view.
PDB entry 3a1p coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Kaminishi T, Takemoto C, Tatsuguchi A, Kawazoe M, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177890 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribosome maturation factor RimM Chains: A, C
Molecule details ›
Chains: A, C
Length: 162 amino acids
Theoretical weight: 18.09 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SJH5 (Residues: 1-162; Coverage: 100%)
Gene names: TTHA1033, rimM
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS19 Chains: B, D
Molecule details ›
Chains: B, D
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SHP2 (Residues: 1-93; Coverage: 100%)
Gene names: TTHA1688, rpsS
Sequence domains: Ribosomal protein S19
Structure domains: 30s Ribosomal Protein S19; Chain A

Ligands and Environments

1 bound ligand:
Ligand UNX 1 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 57.397Å b: 67.491Å c: 62.002Å
α: 90° β: 94.82° γ: 90°
R-values:
R R work R free
0.197 0.197 0.245
Expression system: Escherichia coli BL21(DE3)

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