› 3a1y

X-ray diffraction
2.13Å resolution

The structure of archaeal ribosomal stalk P1/P0 complex

Released:
DOI: 10.2210/pdb3a1y/pdb
PDB entry 3a1y coloured by chain, front view.
PDB entry 3a1y coloured by chain, side view.
PDB entry 3a1y coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Structural basis for translation factor recruitment to the eukaryotic/archaeal ribosomes.
Naganuma T, Nomura N, Yao M, Mochizuki M, Uchiumi T, Tanaka I
J Biol Chem 285 4747-56 (2010)
PMID: 20007716

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129597 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Large ribosomal subunit protein P1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 58 amino acids
Theoretical weight: 6.24 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O57705 (Residues: 1-58; Coverage: 54%)
Gene names: PH1998, rpl12
Sequence domains: 60s Acidic ribosomal protein
Structure domains: Arc Repressor Mutant, subunit A
Large ribosomal subunit protein uL10 Chain: G
Molecule details ›
Chain: G
Length: 284 amino acids
Theoretical weight: 31.16 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O74109 (Residues: 1-284; Coverage: 83%)
Gene names: PH1999, rpl10, rplP0
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 54.88Å b: 104.429Å c: 136.223Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.26
Expression system: Escherichia coli

Quick links

Citations

8 review citations

EF-G and EF4: translocation and back-translocation on the bacterial ribosome.
Yamamoto et al. (2014)
7 more

15 mentions without citation

How Ricin Damages the Ribosome.
Grela et al. (2019)
14 more