› 3a3k

X-ray diffraction
2.5Å resolution

Reversibly bound chloride in the atrial natriuretic peptide receptor hormone-binding domain

Released:
DOI: 10.2210/pdb3a3k/pdb
PDB entry 3a3k coloured by chain, front view.
PDB entry 3a3k coloured by chain, side view.
PDB entry 3a3k coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Reversibly bound chloride in the atrial natriuretic peptide receptor hormone-binding domain: possible allosteric regulation and a conserved structural motif for the chloride-binding site.
Ogawa H, Qiu Y, Philo JS, Arakawa T, Ogata CM, Misono KS
Protein Sci 19 544-57 (2010)
PMID: 20066666

Function and Biology Details

Reaction catalysed: 
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Atrial natriuretic peptide receptor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 435 amino acids
Theoretical weight: 48.43 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P18910 (Residues: 29-463; Coverage: 42%)
Gene name: Npr1
Sequence domains: Receptor family ligand binding region
Structure domains: Rossmann fold

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 4 x NAG
Ligand SO4 9 x SO4
Ligand BR 2 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 120.521Å b: 120.521Å c: 160.613Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.243
Expression system: Cricetulus griseus

Quick links

Citations

5 review citations

Guanylyl cyclase structure, function and regulation.
Potter LR. (2011)
4 more

2 mentions without citation

Reversibly bound chloride in the atrial natriuretic peptide receptor hormone-binding domain: possible allosteric regulation and a conserved structural motif for the chloride-binding site.
Ogawa et al. (2010)
1 more