› 3a43

X-ray diffraction
2.3Å resolution

Crystal structure of HypA

Released:
DOI: 10.2210/pdb3a43/pdb
PDB entry 3a43 coloured by chain, front view.
PDB entry 3a43 coloured by chain, side view.
PDB entry 3a43 coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Crystal structure of HypA, a nickel-binding metallochaperone for [NiFe] hydrogenase maturation.
Watanabe S, Arai T, Matsumi R, Atomi H, Imanaka T, Miki K
J Mol Biol 394 448-59 (2009)
PMID: 19769985

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-177308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrogenase maturation factor HypA Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.74 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JIH3 (Residues: 1-139; Coverage: 100%)
Gene names: TK2008, hypA
Sequence domains: Hydrogenase/urease nickel incorporation, metallochaperone, hypA
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand FME 2 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: R32
Unit cell:
a: 83.933Å b: 83.933Å c: 365.006Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.215 0.239
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Insights into [FeFe]-hydrogenase structure, mechanism, and maturation.
Mulder et al. (2011)
2 more

12 mentions without citation

Specific metal recognition in nickel trafficking.
Higgins et al. (2012)
11 more