› 3a4t

X-ray diffraction
2.3Å resolution

Crystal structure of aTrm4 from M.jannaschii with sinefungin

Released:
DOI: 10.2210/pdb3a4t/pdb
PDB entry 3a4t coloured by chain, front view.
PDB entry 3a4t coloured by chain, side view.
PDB entry 3a4t coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure of Methanocaldococcus jannaschii Trm4 complexed with sinefungin.
Kuratani M, Hirano M, Goto-Ito S, Itoh Y, Hikida Y, Nishimoto M, Sekine S, Bessho Y, Ito T, Grosjean H, Yokoyama S
J Mol Biol 401 323-33 (2010)
PMID: 20600111

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (cytosine(48)-C(5))-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 31.54 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60343 (Residues: 1-274; Coverage: 100%)
Gene names: MJ0026, trm4
Sequence domains: 16S rRNA methyltransferase RsmB/F
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SFG 2 x SFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 170.259Å b: 40.097Å c: 85.951Å
α: 90° β: 110.84° γ: 90°
R-values:
R R work R free
0.186 0.181 0.268
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Methylated nucleosides in tRNA and tRNA methyltransferases.
Hori H. (2014)
1 more

3 mentions without citation

On the evolutionary origin of eukaryotic DNA methyltransferases and Dnmt2.
Jurkowski et al. (2011)
2 more