› 3a52

X-ray diffraction
2.2Å resolution

Crystal structure of cold-active alkailne phosphatase from psychrophile Shewanella sp.

Released:
DOI: 10.2210/pdb3a52/pdb
PDB entry 3a52 coloured by chain, front view.
PDB entry 3a52 coloured by chain, side view.
PDB entry 3a52 coloured by chain, top view.
Source organism: Shewanella sp. AP1
Primary publication:
Crystal structure of cold-active alkaline phosphatase from the psychrophile Shewanella sp.
Tsuruta H, Mikami B, Higashi T, Aizono Y
Biosci Biotechnol Biochem 74 69-74 (2010)
PMID: 20057143

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cold-active alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 43.15 KDa
Source organism: Shewanella sp. AP1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RQU7 (Residues: 1-398; Coverage: 100%)
Gene name: scap
Sequence domains: Alkaline phosphatase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ZN 2 x ZN
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 64.059Å b: 95.863Å c: 71.058Å
α: 90° β: 101.62° γ: 90°
R-values:
R R work R free
0.183 0.183 0.244
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
2 more

5 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
4 more