› 3a79

X-ray diffraction
2.9Å resolution

Crystal structure of TLR2-TLR6-Pam2CSK4 complex

Released:
DOI: 10.2210/pdb3a79/pdb
PDB entry 3a79 coloured by chain, front view.
PDB entry 3a79 coloured by chain, side view.
PDB entry 3a79 coloured by chain, top view.
Source organisms:
Primary publication:
Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer.
Kang JY, Nan X, Jin MS, Youn SJ, Ryu YH, Mah S, Han SH, Lee H, Paik SG, Lee JO
Immunity 31 873-84 (2009)
PMID: 19931471

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-163397 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
LRRNT domain-containing protein; Toll-like receptor 2 Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 65.54 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9QUN7 (Residues: 1-506; Coverage: 63%)
  • Canonical: Q4G1L2 (Residues: 133-200; Coverage: 27%)
Gene names: Tlr2, VLRB
Sequence domains:
LRRNT domain-containing protein; Toll-like receptor 6 Chain: B
Molecule details ›
Chain: B
Length: 562 amino acids
Theoretical weight: 63.79 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9EPW9 (Residues: 1-483; Coverage: 59%)
  • Canonical: Q4G1L3 (Residues: 158-232; Coverage: 27%)
Gene names: Tlr6, VLRB
Sequence domains:
Structure domains: Ribonuclease Inhibitor
Pam2CSK4 Chain: C
Molecule details ›
Chain: C
Length: 6 amino acids
Theoretical weight: 725 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand PXS 1 x PXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6122
Unit cell:
a: 168.901Å b: 168.901Å c: 231.353Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.208 0.28
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

138 review citations

The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors.
Kawai et al. (2010)
137 more

26 mentions without citation

The structural biology of Toll-like receptors.
Botos et al. (2011)
25 more