› 3a7k

X-ray diffraction
2Å resolution

Crystal structure of halorhodopsin from Natronomonas pharaonis

Released:
DOI: 10.2210/pdb3a7k/pdb
PDB entry 3a7k coloured by chain, front view.
PDB entry 3a7k coloured by chain, side view.
PDB entry 3a7k coloured by chain, top view.
Source organism: Natronomonas pharaonis DSM 2160
Primary publication:
Crystal structure of the light-driven chloride pump halorhodopsin from Natronomonas pharaonis.
Kouyama T, Kanada S, Takeguchi Y, Narusawa A, Murakami M, Ihara K
J Mol Biol 396 564-79 (2010)
PMID: 19961859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halorhodopsin Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 291 amino acids
Theoretical weight: 30.98 KDa
Source organism: Natronomonas pharaonis DSM 2160
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

6 bound ligands:
Ligand RET 3 x RET
Ligand 22B 3 x 22B
Ligand L1P 3 x L1P
Ligand L2P 6 x L2P
Ligand L3P 9 x L3P
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 151.83Å b: 99.77Å c: 99.27Å
α: 90° β: 127.67° γ: 90°
R-values:
R R work R free
0.249 0.249 0.274

Quick links

Citations

10 review citations

The development and application of optogenetics.
Fenno et al. (2011)
9 more

24 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
23 more