› 3a7l

X-ray diffraction
1.3Å resolution

Crystal structure of E. coli apoH-protein

Released:
DOI: 10.2210/pdb3a7l/pdb
PDB entry 3a7l coloured by chain, front view.
PDB entry 3a7l coloured by chain, side view.
PDB entry 3a7l coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Global conformational change associated with the two-step reaction catalyzed by Escherichia coli lipoate-protein ligase A.
Fujiwara K, Maita N, Hosaka H, Okamura-Ikeda K, Nakagawa A, Taniguchi H
J Biol Chem 285 9971-9980 (2010)
PMID: 20089862

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine cleavage system H protein Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.69 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6T9 (Residues: 2-129; Coverage: 99%)
Gene names: JW2872, b2904, gcvH
Sequence domains: Glycine cleavage H-protein
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 46.483Å b: 52.037Å c: 46.831Å
α: 90° β: 93.05° γ: 90°
R-values:
R R work R free
0.178 0.176 0.205
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Lipoic acid metabolism in microbial pathogens.
Spalding et al. (2010)
6 more

10 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
9 more