› 3a8n

X-ray diffraction
4.5Å resolution

Crystal structure of the Tiam1 PHCCEx domain

Released:
DOI: 10.2210/pdb3a8n/pdb
PDB entry 3a8n coloured by chain, front view.
PDB entry 3a8n coloured by chain, side view.
PDB entry 3a8n coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The PHCCEx domain of Tiam1/2 is a novel protein- and membrane-binding module.
Terawaki S, Kitano K, Mori T, Zhai Y, Higuchi Y, Itoh N, Watanabe T, Kaibuchi K, Hakoshima T
EMBO J 29 236-50 (2010)
PMID: 19893486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor TIAM1 Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 31.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60610 (Residues: 429-702; Coverage: 17%)
Gene names: Tiam-1, Tiam1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6422
Unit cell:
a: 113.47Å b: 113.47Å c: 113.837Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.306 0.306 0.396
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Rho guanine nucleotide exchange factors: regulators of Rho GTPase activity in development and disease.
Cook et al. (2014)
4 more

5 mentions without citation

Improved low-resolution crystallographic refinement with Phenix and Rosetta.
DiMaio et al. (2013)
4 more