› 3a9q

X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of E173A variant of the soybean ferritin SFER4

Released:
DOI: 10.2210/pdb3a9q/pdb
PDB entry 3a9q coloured by chain, front view.
PDB entry 3a9q coloured by chain, side view.
PDB entry 3a9q coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Crystal structure of plant ferritin reveals a novel metal binding site that functions as a transit site for metal transfer in ferritin.
Masuda T, Goto F, Yoshihara T, Mikami B
J Biol Chem 285 4049-4059 (2010)
PMID: 20007325

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin-4, chloroplastic Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 212 amino acids
Theoretical weight: 23.96 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: Q948P5 (Residues: 36-247; Coverage: 86%)
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand CA 124 x CA
Ligand ACY 18 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21212
Unit cell:
a: 223.503Å b: 221.816Å c: 122.725Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.185
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mechanisms of iron mineralization in ferritins: one size does not fit all.
Bradley et al. (2014)
3 more

2 mentions without citation

Crystal structure of plant ferritin reveals a novel metal binding site that functions as a transit site for metal transfer in ferritin.
Masuda et al. (2010)
1 more