› 3aa4

X-ray diffraction
1.79Å resolution

A52V E.coli RNase HI

Released:
DOI: 10.2210/pdb3aa4/pdb
PDB entry 3aa4 coloured by chain, front view.
PDB entry 3aa4 coloured by chain, side view.
PDB entry 3aa4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Protein core adaptability: crystal structures of the cavity-filling variants of Escherichia coli RNase HI.
Tanaka M, Chon H, Angkawidjaja C, Koga Y, Takano K, Kanaya S
Protein Pept Lett 17 1163-9 (2010)
PMID: 20423323

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HI Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Y4 (Residues: 1-155; Coverage: 100%)
Gene names: JW0204, b0214, dasF, herA, rnh, rnhA, sdrA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 41.472Å b: 85.529Å c: 35.143Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.221
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

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2 more

1 mention without citation

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