› 3aak

X-ray diffraction
2.7Å resolution

Crystal structure of Zn2+-bound form of des3-20ALG-2F122A

Released:
DOI: 10.2210/pdb3aak/pdb
PDB entry 3aak coloured by chain, front view.
PDB entry 3aak coloured by chain, side view.
PDB entry 3aak coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
Inuzuka T, Suzuki H, Kawasaki M, Shibata H, Wakatsuki S, Maki M
BMC Struct Biol 10 25 (2010)
PMID: 20691033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Programmed cell death protein 6 Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 20.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75340 (Residues: 20-191; Coverage: 90%)
Gene names: ALG2, PDCD6
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 71.266Å b: 170.209Å c: 46.911Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.252 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure and function of ALG-2, a penta-EF-hand calcium-dependent adaptor protein.
Maki et al. (2011)
1 more

1 mention without citation

Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
Inuzuka et al. (2010)