› 3ab3

X-ray diffraction
2.4Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151040 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein subunit alpha-13 Chains: A, C
Molecule details ›
Chains: A, C
Length: 362 amino acids
Theoretical weight: 42.36 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P27601 (Residues: 17-43, 47-377; Coverage: 95%)
Gene names: Gna-13, Gna13
Sequence domains: G-protein alpha subunit
Structure domains:
Rho guanine nucleotide exchange factor 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 246 amino acids
Theoretical weight: 27.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92888 (Residues: 1-233; Coverage: 26%)
Gene name: ARHGEF1
Sequence domains: Regulator of G protein signalling-like domain
Structure domains: Regulator of G-protein Signalling 4, domain 2

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GDP 2 x GDP
Ligand ALF 2 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P1
Unit cell:
a: 50.481Å b: 70.64Å c: 88.078Å
α: 77.77° β: 84.45° γ: 80.11°
R-values:
R R work R free
0.205 0.205 0.28
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

Signalling mechanisms of RhoGTPase regulation by the heterotrimeric G proteins G12 and G13.
Kozasa et al. (2011)
2 more

7 mentions without citation

Mutational and structural analysis of diffuse large B-cell lymphoma using whole-genome sequencing.
Morin et al. (2013)
6 more