› 3adf

X-ray diffraction
2.2Å resolution

Crystal structure of a monomeric green fluorescent protein, Azami-Green (mAG)

Released:
DOI: 10.2210/pdb3adf/pdb
PDB entry 3adf coloured by chain, front view.
PDB entry 3adf coloured by chain, side view.
PDB entry 3adf coloured by chain, top view.
Source organism: Galaxea fascicularis
Primary publication:
The structure of mAG, a monomeric mutant of the green fluorescent protein Azami-Green, reveals the structural basis of its stable green emission.
Ebisawa T, Yamamura A, Kameda Y, Hayakawa K, Nagata K, Tanokura M
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 485-9 (2010)
PMID: 20445241

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-178845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric Azami Green Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 26.22 KDa
Source organism: Galaxea fascicularis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60I24 (Residues: 1-225; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 2 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 41.78Å b: 51.72Å c: 52.89Å
α: 90.96° β: 103.41° γ: 101.79°
R-values:
R R work R free
0.208 0.205 0.259
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Condensed genome structure.
Black et al. (2012)
4 other articles

6 mentions without citation

Thermal green protein, an extremely stable, nonaggregating fluorescent protein created by structure-guided surface engineering.
Close et al. (2015)
5 more