› 3adi

X-ray diffraction
3.2Å resolution

Structure of Arabidopsis HYL1 and its molecular implications for miRNA processing

Released:
DOI: 10.2210/pdb3adi/pdb
PDB entry 3adi coloured by chain, front view.
PDB entry 3adi coloured by chain, side view.
PDB entry 3adi coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure of Arabidopsis HYPONASTIC LEAVES1 and its molecular implications for miRNA processing.
Yang SW, Chen HY, Yang J, Machida S, Chua NH, Yuan YA
Structure 18 594-605 (2010)
PMID: 20462493

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-126447 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Double-stranded RNA-binding protein 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 73 amino acids
Theoretical weight: 8.24 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O04492 (Residues: 15-84; Coverage: 17%)
Gene names: At1g09700, DRB1, F21M12.9, HYL1
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
RNA (5'-R(P*CP*UP*CP*GP*AP*UP*AP*AP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.12 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
RNA (5'-R(*GP*GP*UP*UP*AP*UP*CP*GP*AP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 10 nucleotides
Theoretical weight: 3.22 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P43
Unit cell:
a: 47.62Å b: 47.62Å c: 115.377Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.204 0.317
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

Biogenesis, turnover, and mode of action of plant microRNAs.
Rogers et al. (2013)
24 more

7 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
6 more