› 3aeo

X-ray diffraction
2.15Å resolution

Reaction intermediate structure of Entamoeba histolytica methionine gamma-lyase 1 containing methionine alpha, beta-enamine-pyridoxamine-5'-phosphate

Released:
DOI: 10.2210/pdb3aeo/pdb
Model geometry
Fit model/data
PDB entry 3aeo coloured by chain, front view.
PDB entry 3aeo coloured by chain, side view.
PDB entry 3aeo coloured by chain, top view.
Source organism: Entamoeba histolytica
Entry authors: Karaki T, Sato D, Shimizu A, Nozaki T, Harada S

Function and Biology Details

Reaction catalysed: 
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cystathionine gamma-synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 42.35 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86D28 (Residues: 1-389; Coverage: 100%)
Gene name: metg
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 3LM 4 x 3LM
Ligand SO4 4 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 99.057Å b: 85.703Å c: 115.135Å
α: 90° β: 101.39° γ: 90°
R-values:
R R work R free
0.155 0.153 0.194
Expression system: Escherichia coli

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