› 3afb

X-ray diffraction
1.76Å resolution

Crystal structures of catalytic site mutants of active domain 2 of chitinase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb3afb/pdb
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PDB entry 3afb coloured by chain, front view.
PDB entry 3afb coloured by chain, side view.
PDB entry 3afb coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus- the role of conserved residues in the active site.
Tsuji H, Nishimura S, Inui T, Kado Y, Ishikawa K, Nakamura T, Uegaki K
FEBS J 277 2683-95 (2010)
PMID: 20553502

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 34.72 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1H5 (Residues: 409-717; Coverage: 43%)
Gene name: PF1233
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GOL 4 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 89.912Å b: 91.997Å c: 107.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.182
Expression system: Escherichia coli

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Citations

1 review citation

Biodegradation and Prospect of Polysaccharide from Crustaceans.
Qiu et al. (2022)
18 other articles

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