› 3afk

X-ray diffraction
1.95Å resolution

Crystal Structure of Agrocybe aegerita lectin AAL complexed with Thomsen-Friedenreich antigen

Released:
DOI: 10.2210/pdb3afk/pdb
PDB entry 3afk coloured by chain, front view.
PDB entry 3afk coloured by chain, side view.
PDB entry 3afk coloured by chain, top view.
Source organism: Cyclocybe aegerita
Primary publication:
Structural insights into the recognition mechanism between an antitumor galectin AAL and the Thomsen-Friedenreich antigen.
Feng L, Sun H, Zhang Y, Li DF, Wang DC
FASEB J 24 3861-8 (2010)
PMID: 20530247

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Anti-tumor lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 18.18 KDa
Source organism: Cyclocybe aegerita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WY08 (Residues: 1-158; Coverage: 100%)
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: A2G, GAL
Carbohydrate polymer
1 bound ligand:
Ligand THR 2 x THR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P1
Unit cell:
a: 40.85Å b: 43.44Å c: 56.06Å
α: 102.08° β: 98.15° γ: 118.06°
R-values:
R R work R free
0.186 0.186 0.219
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Fungal lectins: structure, function and potential applications.
Varrot et al. (2013)
1 more

2 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
1 more