› 3ahu

X-ray diffraction
2.2Å resolution

Crystal structure of YmaH (Hfq) from Bacillus subtilis in complex with an RNA aptamer.

Released:
DOI: 10.2210/pdb3ahu/pdb
PDB entry 3ahu coloured by chain, front view.
PDB entry 3ahu coloured by chain, side view.
PDB entry 3ahu coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Baba S, Someya T, Kumasaka T, Kawai G, Nakamura K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128371 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein Hfq Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 78 amino acids
Theoretical weight: 8.93 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O31796 (Residues: 1-73; Coverage: 100%)
Gene names: BSU17340, hfq, ymaH
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.
5'-R(*AP*GP*AP*GP*AP*G)-3' Chain: X
Molecule details ›
Chain: X
Length: 6 nucleotides
Theoretical weight: 1.98 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: F222
Unit cell:
a: 91.921Å b: 92.504Å c: 114.918Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.259 0.26
Expression systems:
  • Escherichia coli
  • Not provided

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