› 3ahy

X-ray diffraction
1.63Å resolution

Crystal structure of beta-glucosidase 2 from fungus Trichoderma reesei in complex with Tris

Released:
DOI: 10.2210/pdb3ahy/pdb
PDB entry 3ahy coloured by chain, front view.
PDB entry 3ahy coloured by chain, side view.
PDB entry 3ahy coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
Structural and functional analysis of three β-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei and termite Neotermes koshunensis.
Jeng WY, Wang NC, Lin MH, Lin CT, Liaw YC, Chang WJ, Liu CI, Liang PH, Wang AH
J Struct Biol 173 46-56 (2011)
PMID: 20682343

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 473 amino acids
Theoretical weight: 53.26 KDa
Source organism: Trichoderma reesei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O93785 (Residues: 1-466; Coverage: 100%)
Gene name: bgl2
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand TRS 4 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 92.83Å b: 103.511Å c: 94.802Å
α: 90° β: 105.37° γ: 90°
R-values:
R R work R free
0.167 0.164 0.218
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Bioproducts From Euglena gracilis: Synthesis and Applications.
Gissibl et al. (2019)
5 more

14 mentions without citation

Genomics review of holocellulose deconstruction by aspergilli.
Segato et al. (2014)
13 more