› 3aj4

X-ray diffraction
1Å resolution

Crystal structure of the PH domain of Evectin-2 from human complexed with O-phospho-L-serine

Released:
DOI: 10.2210/pdb3aj4/pdb
PDB entry 3aj4 coloured by chain, front view.
PDB entry 3aj4 coloured by chain, side view.
PDB entry 3aj4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes.
Uchida Y, Hasegawa J, Chinnapen D, Inoue T, Okazaki S, Kato R, Wakatsuki S, Misaki R, Koike M, Uchiyama Y, Iemura S, Natsume T, Kuwahara R, Nakagawa T, Nishikawa K, Mukai K, Miyoshi E, Taniguchi N, Sheff D, Lencer WI, Taguchi T, Arai H
Proc Natl Acad Sci U S A 108 15846-51 (2011)
PMID: 21911378

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pleckstrin homology domain-containing family B member 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 13.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CS7 (Residues: 1-110; Coverage: 50%)
Gene names: EVT2, PLEKHB2
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

2 bound ligands:
Ligand SEP 1 x SEP
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 31.747Å b: 48.449Å c: 64.323Å
α: 90° β: 92.2° γ: 90°
R-values:
R R work R free
0.106 0.105 0.126
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Formation and function of phosphatidylserine and phosphatidylethanolamine in mammalian cells.
Vance et al. (2013)
30 more

4 mentions without citation

Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes.
Uchida et al. (2011)
3 more