› 3aj6

X-ray diffraction
1.48Å resolution

HA1 (HA33) mutant F179I of botulinum type C progenitor toxin complexed with N-acetylgalactosamine, bound at site II

Released:
DOI: 10.2210/pdb3aj6/pdb
PDB entry 3aj6 coloured by chain, front view.
PDB entry 3aj6 coloured by chain, side view.
PDB entry 3aj6 coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Molecular diversity of the two sugar-binding sites of the β-trefoil lectin HA33/C (HA1) from Clostridium botulinum type C neurotoxin.
Nakamura T, Tonozuka T, Ito S, Takeda Y, Sato R, Matsuo I, Ito Y, Oguma K, Nishikawa A
Arch Biochem Biophys 512 69-77 (2011)
PMID: 21640703

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-144104 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Main hemagglutinin component type C Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 33.75 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DPR0 (Residues: 1-286; Coverage: 100%)
Gene names: HA-33, antP-33, ha1
Sequence domains: Ricin-type beta-trefoil lectin domain-like
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand NGA 1 x NGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 137.856Å b: 62.139Å c: 82.336Å
α: 90° β: 104.47° γ: 90°
R-values:
R R work R free
0.168 0.166 0.192
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Botulinum Neurotoxins: Biology, Pharmacology, and Toxicology.
Pirazzini et al. (2017)
5 more

1 mention without citation

Molecular assembly of botulinum neurotoxin progenitor complexes.
Benefield et al. (2013)