› 3ajw

X-ray diffraction
2.1Å resolution

Structure of FliJ, a soluble component of flagellar type III export apparatus

Released:
DOI: 10.2210/pdb3ajw/pdb
PDB entry 3ajw coloured by chain, front view.
PDB entry 3ajw coloured by chain, side view.
PDB entry 3ajw coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Common architecture of the flagellar type III protein export apparatus and F- and V-type ATPases.
Ibuki T, Imada K, Minamino T, Kato T, Miyata T, Namba K
Nat Struct Mol Biol 18 277-82 (2011)
PMID: 21278755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellar FliJ protein Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 17.63 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A1K1 (Residues: 1-147; Coverage: 100%)
Gene names: STM1973, flaO, flaS, fliJ
Sequence domains: Flagellar FliJ protein
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand HG 1 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6522
Unit cell:
a: 52.925Å b: 52.925Å c: 192.597Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.255
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells.
Galán et al. (2014)
27 more

21 mentions without citation

Building a secreting nanomachine: a structural overview of the T3SS.
Abrusci et al. (2014)
20 more