› 3al0

X-ray diffraction
3.37Å resolution

Crystal structure of the glutamine transamidosome from Thermotoga maritima in the glutamylation state.

Released:
DOI: 10.2210/pdb3al0/pdb
PDB entry 3al0 coloured by chain, front view.
PDB entry 3al0 coloured by chain, side view.
PDB entry 3al0 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Ito T, Yokoyama S
Nature 467 612-6 (2010)
PMID: 20882017

Function and Biology Details

Reactions catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-194481 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Glutamyl-tRNA(Gln) amidotransferase subunit A Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 52.59 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X0Z9 (Residues: 1-475; Coverage: 100%)
Gene names: TM_1272, gatA
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B Chain: B
Molecule details ›
Chain: B
Length: 482 amino acids
Theoretical weight: 55.51 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X100 (Residues: 1-482; Coverage: 100%)
Gene names: TM_1273, gatB
Sequence domains:
Structure domains: Arc Repressor Mutant, subunit A
Glutamate--tRNA ligase 2; Glutamyl-tRNA(Gln) amidotransferase subunit C Chain: C
Molecule details ›
Chain: C
Length: 592 amino acids
Theoretical weight: 69.41 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WY94 (Residues: 2-96; Coverage: 99%)
  • Canonical: Q9X2I8 (Residues: 1-487; Coverage: 100%)
Gene names: TM_0252, TM_1875, gatC, gltX2
Sequence domains:
Structure domains:
tRNAGln Chain: E
Molecule details ›
Chain: E
Length: 74 nucleotides
Theoretical weight: 23.86 KDa
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand GSU 1 x GSU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P2221
Unit cell:
a: 76.954Å b: 125.661Å c: 313.386Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.195 0.269
Expression system: Escherichia coli

Quick links

Citations

7 review citations

tRNA biology in mitochondria.
Salinas-Giegé et al. (2015)
6 more

12 mentions without citation

The tRNA Elbow in Structure, Recognition and Evolution.
Zhang et al. (2016)
11 more